The aim of this study was to evaluate the resorption process during the repair of autogenous bone grafts with or without coverage by an expanded polytetrafluoroethylene (e-PTFE) membrane in female rats with estrogen deficiency using the immunohistochemical technique. Eighty female rats were randomly divided into two groups (OVX and SHAM). The 40 female rats in the OVX group were subjected to ovariectomy, and the 40 female rats in the SHAM group were subjected to simulated ovariectomy. The two groups were further divided in subgroup E, which was subjected to surgery for placement of autogenous bone graft (ABG), and subgroup ME, in which the ABG was covered with an e-PTFE membrane. The animals were killed at 0, 7, 21, 45 and 60 days. The specimens were analyzed using immunohistochemistry for the bone resorption markers RANK, RANK-L and Osteoprotegerin (OPG). A higher remodeling rate was observed at 7 and 21 days after the autogenous bone grafts, when the markers were more intensely expressed. At the final time point, the specimens presented similar characteristics to those observed at the initial time point. The expression of immunohistochemical markers was not altered by the estrogen deficiency. The presence of the e-PTFE membrane delayed the bone resorption process, influencing the immunohistochemical expression of markers.

INTRODUÇÃO: A literatura vem relatando condições inadequadas de higiene oral de pacientes em unidade de terapia intensiva (UTI) e a ocorrência de Pneumonia associada à ventilação mecânica em cerca de 9%-27% de todos os pacientes intubados. OBJETIVO: O objetivo deste estudo foi avaliar condições orais de pacientes críticos e correlacionar com a presença de pneumonia associada à ventilação mecânica. MATERIAL E MÉTODO: Vinte e três pacientes foram categorizados da seguinte maneira: com a doença periodontal e com pneumonia associada à ventilação mecânica, com doença periodontal e sem pneumonia associada à ventilação mecânica, sem doença periodontal e com pneumonia associada à ventilação mecânica e sem doença periodontal e sem pneumonia associada à ventilação mecânica. Foram utilizados na avaliação os índices de placa, de doença periodontal e índice de cariados, perdidos e obturados. RESULTADO: Não houve diferença estatisticamente significante na incidência da doença periodontal em relação à pneumonia associada à ventilação mecânica, mas o número de dentes e superfícies com perda de inserção acima de 4 mm sempre foi maior em pacientes com pneumonia associada à ventilação mecânica. CONCLUSÃO: A extensão da doença periodontal pode contribuir para o aparecimento de pneumonia associada à ventilação mecânica. No entanto, são necessários estudos com uma amostra maior para validar essa relação.

INTRODUCTION: Literature has reported inadequate oral hygiene conditions in Intensive Care Unit (ICU) patients and the occurrence of Ventilator-associated pneumonia in about 9%-27% of all intubated patients. OBJECTIVE: The aim of this study was to evaluate ICU patient's oral conditions and correlate this with the presence of ventilator-associated pneumonia. MATERIAL AND METHOD: Twenty-three patients were categorized in the following way: with periodontal disease and ventilator-associated pneumonia, with periodontal disease and without ventilator-associated pneumonia, without periodontal disease and with ventilator-associated pneumonia, and with neither periodontal disease nor ventilator-associated pneumonia. The periodontal disease index, plaque index, and decay-missing-filled index were used in the assessment. RESULT: There was no statistically significant difference in the incidence of periodontal disease with respect to ventilator-associated pneumonia, but the number of teeth and surfaces with attachment loss above 4 mm was always greater in patients with ventilator-associated pneumonia. CONCLUSION: The extent of periodontal disease may contribute to the onset of ventilator-associated pneumonia. However, studies with a larger sample are needed to validate this relationship.